Guideline
 
Microarray Usage Guideline
1.    Users:
Only the NHRI researchers and researchers from the NHRI collaboration research institutes, universities, and industries are eligible for using the service.
2.    Application:
Users can fill the application form online . Before submitting, the application form shall be signed by principal investigators who are responsible to pay for the cost incurred. The application is processed on a first-come-first-service basis.
Cooperation outside the NHRI laboratory to make a user account to be applied beforehand.
Email after completing the application form, please return to the 970305@nhri.org.tw. Only after examination, the laboratory could be apply for jobs online.
 
 
Microarray Usage Guideline > Sample Submission
1.    Users must submit the service application with project/budget account numbers to the Core prior to the third week of each month. The Core will send out the chip orders together on the third week of the month and usually will get the chips and expendable supplies on the second week of the next month. After getting each payment confirmation with the Accounting Office, the Core will begin to conduct each experiment. Had any problem or specific concern regarding the payment of the microarray service, please contact the Core beforehand.
2.    After the application is confirmed, users will receive a notice from the Core and must bring the samples to the Core within the noticed deadline.
3.    Samples Drop-off Day/Time:
Mondays through Fridays 9:00 AM to 5:00 PM
4.    Drop-off Location:
Users shall bring their own samples to the Core at R2-5230 in person. Users working off the Zhunan campus, please submit samples by using low temperature delivery service to the Core. The mailing address is:
R2-5230, 5F, 35 Keyan Road,
Zhunan, Miaoli County 35053, Taiwan
5.    Important Information:
For Gene Expression experiment:
A.   Users must eliminate RNase contamination during the whole process of sample RNAs preparation and add sample RNAs into RNase-free water. It must also be DEPC-free.
B.   Using the Qiagen RNeasy kit to perform RNA isolation and purification is recommended. Another option is using TriZol to isolate RNA first, and then using the Qiagen RNeasy kit to perform RNA isolation and purification (please see the information shown in the link 3 at the “Forms & Links” page).
C.   Please self-check the sample quality first before submitting, such as the OD260/OD280 ratio should be ≧ 1.9, the OD260/OD230 ratio should be ≧ 1.8, and the RNA in agarose gel should show 28S > 18S (see the link 3 at the “Forms & Links” page). After submitting samples, the Core will use the Agilent Bioanalyzer 2100 to run the QC test and will return the unqualified samples and notify the user of the result. In this case, users will be charged for a sample QC fee.
D.   Keep samples in -80°C and ship to the Core with dry ice.
For miRNA Expression experiment:
A.   Do not use any general RNA extraction kit to extract RNAs, which can avoid loss in small RNA.
B.   Please refer to the link 4 at the “Forms & Links” page for the procedure of miRNA extraction and the reagent recommendations.
For Cytogenesis experiment:
A.   DNA sample must be free of contaminants, no heme (from blood), and no chelating agents (EDTA).
B.   The OD260/OD280 ratio should be 1.8 – 2.0 and the OD260/OD230 ratio should be > 1.5.
C.   About 90% of DNA must be longer than 10 kb while running on a 1% agarose gel.
D.   More information about DNA sample preparation please sees the link 5 at the “Forms & Links” page.
6.    Sample Preparation & Sample Quantity:
For RNA QC Service:
The concentration of the sample RNA is 500 ng / μl – 50 ng / μl; the volume should be ≧ 5 μl in a 0.5 ml tube.
   For Gene Expression Assay Service:
The concentration of the sample RNA is 300 ng / μl – 100 ng / μl; the volume should be 5 μl to 10 μl (10 μl is recommended).
   For miRNA Expression Assay Service:
-       Please see the link 4 at the “Forms & Links” page for sample purification method.
-       Total RNA: 0.1 – 3 μg (1 μg is recommended; the concentration is 1 μg /μl) and the volume should be 10 μl.
-       If an enriched sample is not quantitated, use LMW RNA enriched from between 0.1 to 3 µg of total RNA.
NOTE: Suggest using an identical concentration of total RNAs across the same batch of experiments for enrichment. Please refer to the link 4 at the “Forms & Links” page.
    For Cytogenesis Assay Service:
The concentration of human genomic DNA is 33 ng / μl; the total volume should be 10 μl in 1X TE buffer.
 
公告日:2011/07/14





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