Other Services
 
Currently, the Core provides several immuno-staining needed expendable supplies for NHRI researchers to use. There are 3 categories:
  1. Fluorescence Secondary Antibodies
  2. Fluorescence Probes for Immuno-staining
  3. Supplements for Immuno-staining
1.    Fluorescence Secondary Antibodies
After tested in the immunocytochemistry, all antibodies are 100x diluted and aliquoted into 50 μl/vial. It is estimated may supply for more than 25 slide samples staining.
Currently, the 12 different kinds of fluorescence markers are given as follows:
    
1
AMCA-conjugated AffiniPure Donkey Anti-Mouse IgG(H+L)
2
Fluorescein(FITC)-conjugated AffiniPure Donkey Anti-Mouse IgG(H+L)
3
Rhodamine(TRITC)-conjugated AffiniPure Donkey Anti-Mouse IgG(H+L)
4
CyTM5-conjugated AffiniPure Donkey Anti-Mouse IgG(H+L)
5
AMCA-conjugated AffiniPure Donkey Anti-Rabbit IgG(H+L)
6
Fluorescein(FITC)-conjugated AffiniPure Donkey Anti-Rabbit IgG(H+L)
7
Rhodamine(TRITC)-conjugated AffiniPure Donkey Anti-Rabbit IgG(H+L)
8
CyTM5-conjugated AffiniPure Donkey Anti-Rabbit IgG(H+L)
9
AMCA-conjugated AffiniPure Donkey Anti-Goat IgG(H+L)
10
Fluorescein(FITC)-conjugated AffiniPure Donkey Anti-Goat IgG(H+L)
11
Rhodamine(TRITC)-conjugated AffiniPure Donkey Anti-Goat IgG(H+L)
12
CyTM5-conjugated AffiniPure Donkey Anti-Goat IgG(H+L)
 Storage: undissolved antibody should be stored at low temperature in a lyophilized powder form. An undiluted liquid stock can be stored for about 6 months at -80°C. Antibodies will be tested again if exceed the storage date to check for efficiency. The diluted working solution can be kept for about 2 weeks at 4°C.
Usage: undiluted liquid stock can be diluted with PBS/TBS and used on the immune-staining. The diluted solution can be kept at 4°C.
 
2.    Fluorescence Probes for Immuno-staining
After tested immune-staining in the common used cells, all probes are diluted (see below for the recommended dilutions) and aliquoted into 100 μl/vial. It is estimated may supply for more than 25 slide samples staining.
Currently, the 3 different kinds of fluorescence probes are given as follows:
 

1
Nucleus probe – DAPI (1000x)
2
Actin probe – Rhodamine-phalloidin (100x)
3
Actin probe – Oregon Green 488-phalloidin (40x)

Storage: undissolved probes should be stored at lower temperature in a lyophilized powder form. An undiluted liquid stock can be stored for about 3 months at lower temperature. Probes will be tested again if exceed the storage date to check for efficiency. The diluted working solution can be kept at 4°C.
Usage: undiluted liquid stock can be diluted with PBS/TBS and used on the immune-staining. The diluted solution can be kept at 4°C.
 
3.    Supplements for Immuno-staining
The purchase of all supplements for immuno-staining at NOBC has been stopped. Therefore, the stock is currently supplied in a limited amount. Item details are listed below:
 

Item
Description
New user startup set
(limited supply; demand request works in one whole lab as unit)
This set could help the beginners to get into the fluorescence immuno-staining research rapidly. Since this stock is supplied in a limited amount, only the first time fluorescence immuno-staining research user is eligible for making a demand request.
New user startup set includes:
18×18mm cover slip ×1; 22×22mm cover slip ×1; 76×25mm immuno-staining specific slide ×2; 25 slides box ×1; paper slide clip ×1
Paper slide clip
Use for slide storage and can be used as a slide workstation for operating fluorescent staining. Each paper slide clip can store approximately 30 slides.
Mounting media
Use to seal sample slides for storage.
Solutions & Formulas
Include sample fixation buffer, washing buffer, and formula for mounting solution. If in a hurry, user may go and get the already made solutions to use first.

 
 
公告日:2011/07/14





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