PhosphoImager FLA-200 System
 

簡介 使用規則 操作方式

 PhosphoImager FLA-200 System  聯絡人: 林志熙 | 分機: 35341
簡介
 

本機可補捉放射線與螢光兩種訊號,最大樣本尺寸為20X40cm簡介如下:

I. 螢光影像的補捉(Capturing Fluorescent Images)


本機配備有兩個雷射激發光源,分別為SHG laser at 473 nm and a He-Ne laser at 633 nm, 並配備有Y520, O580, R675 三種filter,其精確度可達每像素50um, 且不論Gel厚薄,其雷射強度均可穿透。可應用的螢光物質初步整理如下表:

Excitation

Emission

Chemicals

473nm

Y520

FITC, Cy(TM) 2, SYBR(R) Green I & II, SYPRO(R) Orange, AttoPhos(TM), and others.

473nm

O580

EtBr, PE(phycoerythrin)

633nm

R675

Cy5, DDAO phosphate and others

II. 放射線影像的補捉(Capturing Radioisotope Images)


隨機附有2片Image Plate(IP),其上附著有5um結晶大小的BaFBr: Eu2+的粒子,這些粒子會補捉放射線能量,並隨後在雷射掃描時放出冷光(luminescence),其解析度可達5 to 40 pixels/mm。使用後IP再以日光燈進行抹除(Erase)後重覆使用。以下列舉幾個特性以與X-ray autoradiography 方法做個比較。

  1. Ultrahigh sensitivity. Several ten times more sensitive than film, and several thousand times depending on the sample.
  2. Wider dynamic range. A wider range of 104 to 105 over the 102 range of the photographic method.
     
  3. Superior linearity. The fluorescence emission is proportional to the dose in the entire range.
     
  4. Higher spatial resolution. When compared with other electronic systems, a higher pixel density can be designed to meet the system purpose though less freely than film.
     
  5. Digital electric signals are directly available from the reader. Computer processing or combination with other electronic systems is easy.
     
  6. Due to an integral-type detector, the IP method produces less detection counting errors even at a high flux density, which often happen with pulse-type detectors such as the proportional counter tube and scintillation counter.
     
  7. The accumulated background can be erased before use.
     
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使用規則
 
  • 初次使用者請與基因組管理人聯絡(35341),我們會在詢問與確定使用者能操作無誤後發給必要的配件後開始使用。
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操作方式
 

螢光用平板 放射線用平板

Image Plate(IP板)

 

開機器電源→開電腦主機→

如屬螢光在如圖示的玻璃舖上一層保鮮膜後,將平板以正確方向放入→

開啟軟体進行掃描→ 定量分析

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公告日:2011/07/14





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